Fluorescence wide-field and confocal microscopy localization of polyphenols in plant tissues

Multispectral fluorescence microimaging and microspectrofluorimetry represent useful tools to localize polyphenolic compounds in plant tissues. UV-excited blue autofluorescence can be used as proxy of hydroxycinnamic acids, while flavonoids can be detected as yellow fluorescence under blue excitation, once treated the sample with a specific fluorescence enhancer.

The information obtained are fundamental in eco-physiology studies to elucidate and understand the multifunctional role covered by these compounds in plants under different environmental conditions.

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