Advanced methods in laser biomedical imaging

We have developed new non-invasive imaging and diagnostic techniques based on pulsed lasers for applications in biomedicine:

  1. Multiphoton microscopy and in particular second harmonic generation (SHG) microscopy, which is a novel tool based on nonlinear light scattering. SHG imaging proves to be ideal to investigate pathological conditions of connective tissue such as the cornea and gain insight into the distribution and conformation of biological macromolecules of clinical relevance (in collaboration with INO and LENS).
  2. Photoacoustic (PA) microscopy and tomography for a variety of biomedical applications, including early detection of cancer (in collaboration with IFC). These methods rely on the analysis of ultrasounds that are generated by the rapid photothermal heating and thermoelastic expansion occurring upon absorption of pulsed light. The combination of optical excitation and acoustic detection allows one to reach far deeper penetration into biological tissue than wholly optical imaging techniques, while maintaining the high contrast and spectroscopic specificity of optical imaging. In this context, we are also testing the use of plasmonic particles as contrast agents to increase the sensitivity and spectroscopic specificity of PA signals.

For more info please contact Fulvio Ratto Questo indirizzo email è protetto dagli spambots. È necessario abilitare JavaScript per vederlo. or Lucia Cavigli Questo indirizzo email è protetto dagli spambots. È necessario abilitare JavaScript per vederlo. or Roberto Pini Questo indirizzo email è protetto dagli spambots. È necessario abilitare JavaScript per vederlo.